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Fluorescent Probes, Labels, Particles and Stains > Fluorescent Enzyme Substrates > Alphabetical
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Enzyme Substrates > Substrates by Enzyme > Glucuronidase, beta-

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69602

Sigma

4-Methylumbelliferyl-β-D-glucuronide hydrate

BioChemika, for fluorescence, ≥99.0% (HPLC)


Synonym:	MUG, 4-Methylumbelliferyl-β-D-glucopyranosiduronic acid
CAS Number:	881005-91-0
Linear Formula:	C₁₆H₁₆O₉ · xH₂O
Molecular Weight:	352.29 (anhydrous basis)
EC Number:	228-186-0
MDL number:	MFCD09039280
PubChem Substance ID:	24885956

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Description

Application	suitable as fluorogenic substrate for β-glucuronidase
Other Notes	Substrate for β-D-glucuronidase assay. Has been used in a method to measure liposome lysis by n-octylglucoside¹

Properties

grade	for fluorescence
product line	BioChemika
assay	≥99.0% (HPLC)
total impurities	≤0.1% free 4-Methylumbelliferone
	≤15% water
fluorescence	λ_ex 365 nm; λ_em 445 nm in 0.1 M phosphate pH 6.5
solubility	DMSO: 50 mg/mL, clear, colorless to very faintly yellow
storage temp.	−20°C

Safety

Personal Protective Equipment	Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
WGK Germany	3

References

Cited Reference

1. R.B. Thompson, B. Richard Anal. Lett. 18, 1847, (1985)

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